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KMID : 0811720110150050251
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Korean Journal of Physiology & Pharmacology
2011 Volume.15 No. 5 p.251 ~ p.258
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Lactosylceramide Mediates the Expression of Adhesion Molecules in TNF-? and IFN?-stimulated Primary Cultured Astrocytes
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:Lee Jin-Koo
:Kim Jin-Kyu/:Park Soo-Hyun/:Sim Yun-Beom/:Jung Jun-Sub/:Suh Hong-Won
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Abstract
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Here we have investigated how lactosylceramide (LacCer) modulates gene expression of adhesion molecules in TNF-? and IFN?(CM)-stimulated astrocytes. We have observed that stimulation of astrocytes with CM increased the gene expression of ICAM-1 and VCAM-1. D-Threo-1-phenyl- 2-decanoylamino-3-morpholino-1-propanol (PDMP) and N-butyldeoxynojirimycin (NBDNJ), inhibitors of glucosylceramide synthase (GLS) and LacCer synthase (galactosyltransferase, GalT-2), inhibited the gene expression of ICAM-1 and VCAM-1 and activation of their gene promoter induced by CM, which were reversed by exogenously supplied LacCer. Silencing of GalT-2 gene using its antisense oligonucleotides also attenuated CM-induced ICAM-1 and VCAM-1 expression, which were reversed by LacCer. PDMP treatment and silencing of GalT-2 gene significantly reduced CM-induced luciferase activities in NF-?B, AP-1, GAS, and STAT-3 luciferase vectors-transfected cells. In addition, LacCer reversed the inhibition of NF-?B and STAT-1 luciferase activities by PDMP. Taken together, our results suggest that LacCer may play a crucial role in the expression of ICAM-1 and VCAM-1 via modulating transcription factors, such as NF-?B, AP-1, STAT-1, and STAT-3 in CM-stimulated astrocytes.
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KEYWORD
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ICAM-1, VCAM-1, D-Threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, Lactosylceramide, Astrocytes
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